Biochemical and molecular analysis of glutamine synthetase gene in brassica napus L.
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Chloroplastic glutamine synthetase from Brassica napus.
In higher plants, the GS/glutamate synthase cycle provides the only efficient pathway for the conversion of inorganic nitrogen to the organic form (Stewart et al., 1980). In this connection, GS catalyzes the ATP-dependent condensation of ammonia with glutamate to yield Gln. Ammonia, the ultimate inorganic form of nitrogen available to the plant, is released by a variety of physiological process...
متن کاملSome Biochemical Parameters of Brassica napus L
s: Chromium is one of the unessential heavy metal that makes high toxicity in the plants. The aim of this study was assaying the changes that come from toxicity of (Cr ) on some biochemical, physiological and 6+ ionic specification of Brassica napus variety sari flower. The tests were conducted at hydro ionic conditions and at 0, 25, 50, 75 and 100 μm concentration of K CrO with four repeats in...
متن کاملMolecular Composition of Glutamine Synthetase of Sinapis alba L
Matthias Höpfner , Georg Reifferscheid, and Aloysius Wild Institut für Allgemeine Botanik der Johannes Gutenberg-Universität, Saarstraße 21, D-6500 Mainz, Bundesrepublik Deutschland Z. Naturforsch. 43c, 194-198 (1988); received November 26, 1987 Active Centers, Enzyme Purification, Enzyme Structure, Glufosinate, Glutamine Synthetase, Phosphinothricin Chloroplastic glutamine synthetase of Sinapi...
متن کاملبررسی تغییرات کمّی و کیفی میزان پروتئین، کلروفیل و کاروتنوئید در کلزای تراریخت شده با آنتی سنس ژن گلوتامین سنتتاز (GS1)
Analysis of transgenic plants is very important in gene transfer programs. In this research, the second generation (T1) of transgenic brassica napus which was transformed by antisense of Glutamine synthetase (GS) gene was studied from the view of total soluble protein content of leaf, total chlorophyll and protein patterns (SDS-PAGE) using seeds of Brassica napus .Protein concentration was dete...
متن کاملبررسی تغییرات کمّی و کیفی میزان پروتئین، کلروفیل و کاروتنوئید در کلزای تراریخت شده با آنتی سنس ژن گلوتامین سنتتاز (GS1)
Analysis of transgenic plants is very important in gene transfer programs. In this research, the second generation (T1) of transgenic brassica napus which was transformed by antisense of Glutamine synthetase (GS) gene was studied from the view of total soluble protein content of leaf, total chlorophyll and protein patterns (SDS-PAGE) using seeds of Brassica napus .Protein concentration was dete...
متن کاملFunctional Analysis of the Brassica napus L. Phytoene Synthase (PSY) Gene Family
Phytoene synthase (PSY) has been shown to catalyze the first committed and rate-limiting step of carotenogenesis in several crop species, including Brassica napus L. Due to its pivotal role, PSY has been a prime target for breeding and metabolic engineering the carotenoid content of seeds, tubers, fruits and flowers. In Arabidopsis thaliana, PSY is encoded by a single copy gene but small PSY ge...
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عنوان ژورنال
دوره 20 شماره 1
صفحات 17- 36
تاریخ انتشار 2622-06-22
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